Treatment of cats with feline infectious peritonitis

Feline infectious peritonitis (FIP) infection resulting in clinical signs is invariably fatal despite clinical intervention. As FIP is an immune-mediated disease, treatment is mainly aimed at controlling the immune response triggered by the infection with the feline coronavirus (FCoV). Immune suppressive drugs such as prednisone or cyclophosphamide may slow disease progression but do not produce a cure. In nearly every published case report of attempted therapy for clinical FIP, glucocorticoids have been used; there are, however, no controlled studies that evaluate the effect of glucocorticoids as a therapy for FIP. Some veterinarians prescribe immune modulators to treat cats with FIP with no documented controlled evidence of efficacy. It has been suggested that these agents may benefit infected animals by restoring compromised immune function, thereby allowing the patient to control viral burden and recover from clinical signs. However, a non-specific stimulation of the immune system may be contraindicated as clinical signs develop and progress as a result of an immune-mediated response to the mutated FCoV.     

Substituted alpha-(phenylhydrazono)phenylacetonitrile derivatives. Part 2: Synthesis and biological activity of pro-pesticides

Pro-pesticides of alpha-(2,6-dichloro-4-trifluoromethylphenylhydrazono)-4-nitrophenylacetonitrile have been prepared and tested against mite and insect pests. Variations in potency and spectrum were observed depending on the choice of cleavable pro-moiety. Cleavage of the pro-moiety was demonstrated in one case by measuring the rate of increase in the uncoupling activity using a mitochondrial preparation. Irradiation experiments have demonstrated a rapid isomerisation of the planar Z isomer to the E isomer, which is reversible.     

Glycoside Hydrolase Family 51 α‐l‐Arabinofuranosidases from Clostridium thermocellum and Cellvibrio japonicus Release O–5‐Feruloylated Arabinose

Arabinofuranosidases act synergistically with other enzymes to depolymerize arabinoxylans by cleaving arabinofuranose substituents from the β‐(1→4)‐linked d ‐xylopyranose backbone. Because arabinose feruloylation is a barrier to some, but not all, arabinofuranosidases, we investigated the actions of three α‐l ‐arabinofuranosidases from the glycoside hydrolase (GH) family 51 on feruloylated arabinoxylan‐oligosaccharide standard compounds with and without feruloyl esterase. GH51 α‐l ‐arabinofuranosidases from Clostridium thermocellum and Cellvibrio japonicus both partially released feruloylated arabinose (up to 59% for C. thermocellum). Simultaneous incubation with arabinofuranosidases and feruloyl esterase quantitatively released arabinose from feruloylated standard compounds. Therefore, although feruloylation does not completely obstruct GH51 arabinofuranosidases, synergistic approaches utilizing multiple enzymes remain the most effective tactic for enzymatic breakdown of feruloylated compounds.     

Anatomical differences in the structural elements of fluid passage of Scots pine sapwood with contrasting treatability

Treatability of wood is a function of anatomical properties developed under certain growing conditions. While Scots pine sapwood material normally is considered as easy to impregnate, great variations in treatability can be observed. In order to study anatomical differences in the structural elements of transverse fluid passage, wood material with contrasting treatability has been compared. Ray composition and resin canal network, membrane areas of fenestriform pits in the cross-field as well as dimension and properties of bordered pits were investigated. The results showed large anatomical differences between the two contrasting treatability groups. Refractory Scots pine sapwood samples developed more rays per mm² tangential section, while they were on average lower in cell numbers than rays found in easily treatable material. Easily treatable material had more parenchyma cells in rays than refractory material. At the same time, a larger membrane area in fenestriform pits in the cross-field was observed in the easily treatable sample fraction. Differences in the composition of resin canal network were not observed. Refractory samples developed on average smaller bordered pit features, with relatively small formed pit apertures compared to the easily treatable samples. In refractory Scots pine sapwood material, the structural elements of fluid passage such as bordered pit dimensions, fenestriform pits in the cross-field and parenchyma cells were altogether developed in smaller dimensions or number. Wood samples from better growing conditions and sufficient water supply showed a better treatability in this study.     

Variation in treatability of Scots pine sapwood: a survey of 25 different northern European locations

The treatability of Scots pine sapwood sampled from 25 locations in northern Europe, which was impregnated with an experimental furfuryl alcohol mix, was studied. A large variation in treatability was found between stands. The treatability was affected by anatomical properties of the trees and therefore also by the immediate climate, the sociological position of the tree in the stand, growth increments and on-stand competition. With the models applied, most parts of the differences in treatability between stands could be explained, while the treatability variation between trees within a stand remained mostly unexplained. Wider annual rings and higher latewood contents were found to increase the treatability. Models that included data on growth conditions and climate explain more of the variation in treatability, indicating that also other anatomical properties are influencing the treatability. Tree attributes and growth conditions that reduce annual ring width and latewood content decreased the treatability. Trees growing under warmer conditions and developing larger growth increments were easier to impregnate. Wood from trees growing near the timberline and under environmental conditions impairing wood growth was more difficult to treat.     

IMAGING DIAGNOSIS—CONVENTIONAL AND FUNCTIONAL MAGNETIC RESONANCE IMAGING OF A BRAIN ABSCESS IN A GOAT

A 2‐month‐old female goat was presented for depressed mental status and multifocal central neurologic signs 3 weeks after hot‐iron disbudding. Conventional magnetic resonance imaging (MRI) findings included a large intra axial mass in the left frontal lobe that was T2 hyperintense and T1 hypointense centrally with a contrast‐enhancing peripheral capsule and perilesional T2 hyperintensity. A restrictive pattern was present in diffusion‐weighted imaging. Magnetic resonance spectroscopy demonstrated an increased amount of succinate, acetate, amino acids, lipids; minimal amounts of lactate; and decreased amounts of N‐acetyl aspartate and choline. A cerebral abscess due to Trueperella pyogenes was confirmed from necropsy and tissue culture.     

Prevalence of antibodies against feline panleukopenia virus in client-owned cats in Southern Germany

Feline panleukopenia is a frequent and commonly fatal disease of cats. Recent published studies have raised suspicions that some cats fail to develop antibodies after vaccination. The purpose of this study was to assess the prevalence of antibodies against feline panleukopenia virus (FPV) in cats in Southern Germany, and to identify factors that are associated with a lack of antibodies. In total, 350 cats presented to the Clinic of Small Animal Medicine, Ludwig-Maximilians-Universitaet were randomly included in the study. Information regarding signalment, origin, environment, lifestyle, housing conditions, health status, chronic diseases, glucocorticoid therapy, and vaccination status were collected. Antibodies were detected by haemagglutination inhibition test. Asymptomatic chi-squared tests and univariable logistic regression were used to investigate associations between a lack of antibodies and the different variables. Associations determined to be statistically significant at P < 0.1 were verified by a multivariable logistic regression analysis.Of the 350 cats, 103 (29.4%) had no antibodies against FPV. Chronic kidney disease, neoplasia, glucocorticoid therapy, and vaccination status were significantly associated with a lack of antibodies. The cats with no antibodies were likely to have inadequate immunity against panleukopenia and those with chronic diseases or receiving glucocorticoids were less likely to be protected.     

Nucleus accumbens D2/3 receptors predict trait impulsivity and cocaine reinforcement

Stimulant addiction is often linked to excessive risk taking, sensation seeking, and impulsivity, but in ways that are poorly understood. We report here that a form of impulsivity in rats predicts high rates of intravenous cocaine self-administration and is associated with changes in dopamine (DA) function before drug exposure. Using positron emission tomography, we demonstrated that D2/3 receptor availability is significantly reduced in the nucleus accumbens of impulsive rats that were never exposed to cocaine and that such effects are independent of DA release. These data demonstrate that trait impulsivity predicts cocaine reinforcement and that D2 receptor dysfunction in abstinent cocaine addicts may, in part, be determined by premorbid influences.     

Ribosome assembly factors prevent premature translation initiation by 40S assembly intermediates

Ribosome assembly in eukaryotes requires approximately 200 essential assembly factors (AFs) and occurs through ordered events that initiate in the nucleolus and culminate in the cytoplasm. Here, we present the electron cryo-microscopy (cryo-EM) structure of a late cytoplasmic 40S ribosome assembly intermediate from Saccharomyces cerevisiae at 18 angstrom resolution. We obtained cryo-EM reconstructions of preribosomal complexes lacking individual components to define the positions of all seven AFs bound to this intermediate. These late-binding AFs are positioned to prevent each step in the translation initiation pathway. Together, they obstruct the binding sites for initiation factors, prevent the opening of the messenger RNA channel, block 60S subunit joining, and disrupt the decoding site. These redundant mechanisms probably ensure that pre-40S particles do not enter the translation pathway, which would result in their rapid degradation.